Our company stands at the forefront of preformulation, we can offer cutting-edge services in the determination of pKa values. With many years of experience in the field, we have cultivated unparalleled expertise and can support our customers' formulation research. If you have any needs, please feel free to contact us.
Our Services
The dissociation constant (pKa) determines the degree of solubility and ionization in a pH-dependent environment. The degree of ionization determines absorption as only the ionized form can be absorbed, therefore determining the pKa value of a molecule becomes essential. pKa value measurements provide information about drug absorption, with weakly acidic drugs with a pKa value of approximately 4 being most readily absorbed from the stomach and basic drugs with a pKa value of approximately 8 being most readily absorbed from the gut. The percentage of ionization can be determined by the following equation:
At our company, we have a team of experienced experts dedicated to providing customers with accurate and reliable drug pKa determination services to help guide the optimization of pharmaceutical formulations. We use a variety of advanced analytical techniques, including UV-Vis spectroscopy, nuclear magnetic resonance spectroscopy, fluorescence spectroscopy, high-performance liquid chromatography, and capillary electrophoresis, to ensure that the pKa value of your drug molecules can be accurately determined.
Our Methods
| UV-Vis spectroscop | This technique obtains a molecule's absorption spectrum by measuring the absorption of ultraviolet and visible light by a substance. The pKa value can be determined by monitoring the change in the absorption peak of the acid-base indicator at different pH values. |
| NMR spectroscopy | NMR technology can obtain molecular structure and environmental information by analyzing the resonance signals of atomic nuclei such as hydrogen and carbon in molecules. By monitoring the chemical shift as a function of pH, the pKa value can be inferred. |
| Fluorescence spectroscopy | Fluorescence spectroscopy can measure the fluorescent light signal emitted by molecules after excitation, so as to obtain molecular structure and environmental information. The change in the emission spectrum of fluorescent dyes at different pH values can be used to determine the pKa value. |
| HPLC | HPLC can be used to separate and analyze components in complex mixtures. By analyzing the retention time of drug molecules under different pH conditions, information related to the pKa value can be obtaine |
| Capillary electrophoresis | Capillary electrophoresis can determine the charge state of a drug molecule by analyzing its migration velocity in an electric field, thereby inferring the pKa value. |
Advantages
- Expertise and experience: Our company has many years of experience in the field of preformulation and pKa determination. This expertise allows them to offer accurate and reliable pKa determination services, ensuring that customers receive high-quality results.
- Comprehensive approach: Our company utilizes multiple techniques for pKa determination, increasing the robustness and reliability of the results. This comprehensive approach ensures that the pKa value is accurately determined from different perspectives, enhancing the overall accuracy of the analysis.
Delivery
Complete experimental data are provided.
Please kindly note that our services are for research use only.
