Our company is committed to preformulation study and has extensive experience in this field. We can provide you with high-quality partition coefficient determination services to assist you in choosing the right solvent for the formulation, as well as to study the binding capacity of the drug to serum albumin and the permeability of the drug across biological membranes. If you have any needs, please feel free to contact us.
Our Services
The partition coefficient (Log P) value was defined as the ratio of unionized drugs partitioned between the aqueous and organic phases. The oil-water partition coefficient reflects the ability of a drug to cross a lipid membrane. The lipophilic/hydrophilic balance is one of the most important influencing factors for optimal drug absorption and delivery. Due to the lipid nature of biological membranes, the amount of drug absorbed depends largely on their lipophilicity.
A Log P value of 0 indicates that the drug is evenly distributed in both the water and the partition solvent. Log P values less than 1 indicate higher water solubility, while values greater than 1 indicate higher fat solubility. For optimal solubility and absorption, a proper hydrophilic-lipophilic balance is necessary.
At our company, with our state-of-the-art equipment and specialized technical staff, we can measure the partition coefficient logP of APIs to evaluate its ability to be absorbed across the lipid bilayer in vivo and its pharmacokinetics using reversed-phase high-performance liquid chromatography (RP-HPLC), and shake flasks.
Our Methods
| Shake flask method | The shake flask method is to shake a flask containing two immiscible solvents and a solute. After reaching equilibrium, analyze the concentration of the solute in one or both phases, thereby calculating logP. The shake flask method usually utilizes an octanol-water system to determine LogP. Octanol was chosen as the partitioning solvent for several reasons:
Octanol is thought to mimic the lipidic properties of biological membranes because it contains a polar head and a nonpolar tail.
Octanol is an organic compound that is immiscible in water.
Most drugs have solubility parameters similar to octanol. |
| RP-HPLC | Reverse-phase high-performance liquid chromatography has the advantages of simple operation, rapidity, low cost, and good repeatability, and is a good method for indirect determination of drug logP. |
Advantages
- Diversified assay methods: Our company provides a variety of assay methods to meet the needs of different drug molecules. This allows customers to choose the most suitable measurement method according to the actual situation.
- Advanced equipment and technology: Our company is equipped with advanced experimental equipment, which can efficiently and accurately determine the lipid-water partition coefficient to provide reliable experimental results for drug development and optimization.
- Data interpretation and reporting: In addition to providing experimental data, our company is also able to interpret experimental results and provide detailed reports for customers. This helps customers better understand the properties of drug molecules, thereby guiding subsequent drug development.
- Fast response and delivery: Our company is known for its efficient way of working, being able to quickly respond to customer needs, and providing timely experimental results.
Delivery
Complete experimental data are provided, such as partition coefficients of drug molecules in different phases (e.g. n-octanol and water), resulting in corresponding LogP values.
Please kindly note that our services are for research use only.
